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Science · Grade 7 Science

Chapter 1: Cells: The Unit of Life

Using a Microscope

Magnification is not the same as resolution.

Lesson
4
Time
About 20 minutes
Practice
Planning and carrying out investigations
Crosscutting concept
Scale, proportion, and quantity
Core idea
LS1.A: Structure and Function

The Blurry Bacteria

Sam puts a smear of yogurt under the school microscope at 400×. The bacteria in it show up only as tiny grey specks.

Would more magnification show what the bacteria really look like?

Page 1

Make your guess to turn the page.

Still to come: 10 pages · Words to Know · What We Learned · 4 questions · Explain It in Writing · Ask and Wonder

For the grown-up

The idea this lesson is working on — Children treat the magnification number as the measure of a microscope, because bigger feels like better. The test slide turns detail into a count, and the screen zoom multiplies the size by 4 while the count stays at 4. Ask: what changed between 1,000× and 4,000×, and what did not? The idea to reach is that magnification sets size and resolution sets detail.

Why the stronger objectives also resolve better — Resolution depends on the wavelength of light and on the numerical aperture of the objective, roughly how wide a cone of light it collects. Higher-power objectives are made with larger apertures, and the 100× oil-immersion lens reaches about 0.0002 mm, so detail rises with objective power up to about 1,000×. Beyond that, magnification is "empty". Technique still matters: start at low power, focus with the coarse knob, then switch up and use fine focus only.

About the numbers — Visible light spans roughly 0.0004 to 0.0007 mm in wavelength; the best light microscopes resolve about half of that, near 0.0002 mm. Lactobacillus rods in yogurt are about 0.5–1 micrometer wide and a few micrometers long. The test-slide counts are an illustration consistent with typical 10×, 40× and 100× objectives, not a published measurement.